APPswe Stable Expression Cell Line (SH-SY5Y)

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  • 细胞图0 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图0
  • 细胞图1 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图1
  • 质粒图0 APPswe Stable Expression Cell Line (SH-SY5Y)-质粒图0
  • APPswe Stable Expression Cell Line (SH-SY5Y) APPswe Stable Expression Cell Line (SH-SY5Y)-产品图
细胞图0 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图0
细胞图1 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图1
质粒图0 APPswe Stable Expression Cell Line (SH-SY5Y)-质粒图0
APPswe Stable Expression Cell Line (SH-SY5Y) APPswe Stable Expression Cell Line (SH-SY5Y)-产品图
APPswe Stable Expression Cell Line (SH-SY5Y) Copy Product Info
Catalog#： YOE-L100
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图0
  • 细胞图1 APPswe Stable Expression Cell Line (SH-SY5Y)-细胞图1
  • 质粒图0 APPswe Stable Expression Cell Line (SH-SY5Y)-质粒图0
  • APPswe Stable Expression Cell Line (SH-SY5Y) APPswe Stable Expression Cell Line (SH-SY5Y)-产品图
APPswe Stable Expression Cell Line (SH-SY5Y) Copy Product Info
Catalog#： YOE-L100
Size： 1*10^6
Culture method： 90%DMEM+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L100 Transcript NM_201414.2
Cell name APPswe Stable Expression Cell Line (SH-SY5Y) Species Human
Gene Name APPswe Passage ratio 1:2-1:3
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=2μg/ml
Culture method 90%DMEM+10% FBS Mycoplasma test negative
Catalog
YOE-L100
Cell name
APPswe Stable Expression Cell Line (SH-SY5Y)
Gene Name
APPswe
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Transcript
NM_201414.2
Species
Human
Antibiotic Concentration
Puro=2μg/ml
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS

Product Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
Gene Expression Validation
No. Sample APPswe-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 APPswe Stable Expression Cell Line (SH-SY5Y) 16.481 15.098 1.383 -1.829 3.553
2 SH-SY5Y_Ctrl(Puro) 20.082 16.87 3.212 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:SH-SY5Y		 STR Info (Cell bank)
Cell Line:SH-SY5Y
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 710710
D16S539 813813
VWA 14181418
TH01 710710
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 1822
FGA 23.224
D2S1338 1719
D21S11 3131.2
D18S51 1316
D8S1179 1515
D3S1358 1516
D6S1043 1218
PENTAE 711
D19S433 1314
PENTAD 1012
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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